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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: The whole profiling and competing endogenous RNA network analyses of noncoding RNAs in adipose-derived stem cells from diabetic, old, and young patients

Fig. 7

PCR analysis of RNAs in the above PPI and ceRNA networks, and construction of ceRNA sub-networks. a, b PCR validation of the selected mRNAs in O-ASCs vs. Y-ASCs and D-ASCs vs. O-ASCs. N = 5. c, d PCR validation of the selected lncRNAs and circRNAs in O-ASCs vs. Y-ASCs and D-ASCs vs. O-ASCs. N = 5. e, f Construction of ceRNA sub-networks based on the above PCR results. g–i Correlation analysis of the expression level of miR-145-5p with the expression levels of RAET1E-AS1, WNT11, and BMPER. N = 13-15. j, k Correlation analysis of the expression level of RAET1E-AS1 with the expression levels of WNT11 and BMPER. N = 15, 17. l PCR analysis of the expressional changes of RNAs in the ceRNA sub-network after miR-145-5p overexpression in ASCs. *p< 0.05, **p< 0.01, ***p< 0.001, ****p< 0.0001

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Stem Cell Research & Therapy

ISSN: 1757-6512