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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Exosomes from miRNA-378-modified adipose-derived stem cells prevent glucocorticoid-induced osteonecrosis of the femoral head by enhancing angiogenesis and osteogenesis via targeting miR-378 negatively regulated suppressor of fused (Sufu)

Fig. 4

miR-378 promoted angiogenesis and osteogenesis by down-regulating Sufu and inducing Shh activity. a The binding sites of miR-378 and Sufu were validated using an online website. b The targeting relationship between miR-378 and Sufu was verified suing dual luciferase reporter gene assay. c Expression patterns and extent of Shh signaling pathway-related factors (Sufu, Ptch1, and Gli1) identified by Western blot analysis. d Tube formation assay for detecting tube-forming ability of HUVECs in the different groups. e Expression of ALP was determined by ALP staining in different groups. f Expression patterns of vascular endothelial growth factors (ANG-1, VEGF) and osteogenesis-related factors (BMP2, RUNX2) were measured by Western blot analysis. g Western blotting of Ptch1 and Gli1 expression. *P < 0.05, **P < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512