Fig. 3

IL-1β-primed ERCs reduced Th1 and Th17, but enhance Th2 and Treg populations in colitis mice. Splenocytes were collected on the 10th day after DSS induction. To accurately identify the subpopulation of Th1, Th2, and Th17 cells, splenocytes were firstly incubated with a cell stimulation cocktail for 5 h before being stained with fluorescent antibodies. A Representative dot plots of CD4⁺IFN-γ⁺ Th1 cells, CD4⁺IL-4⁺ Th2 cells, and CD4⁺IL-17⁺ Th17 cells were shown while positive cells were counted from the quadrant Q2. B Dot plots of CD4⁺CD25⁺Foxp3⁺ Tregs. C Percentage of CD4⁺IFN-γ⁺ Th1 cells. D Percentage of CD4⁺IL-4⁺ Th2 cells. E Percentage of CD4⁺IL-17⁺Th17 cells. F Percentage of CD4⁺CD25⁺Foxp3⁺ Tregs. Data were mean ± SD (n = 6, *p < 0.05, **p < 0.01, ***p < 0.001). p values were calculated by one-way ANOVA followed by the LSD test