Fig. 4

IL-1β-primed ERCs reduced the population of mature DCs, but increased M2 macrophages in colitis mice. To determine whether each treatment has an influence on regulating DC and macrophage phenotypes, anti-CD11c antibody and antigen presenting-related antibodies (anti-MHCII, anti-CD86) were used to measure mature DCs, while anti-CD68 antibody and anti-CD206 antibody were used for M2 phenotype macrophages in spleens. A Representative dot plots of CD11c⁺MHCII⁺ DCs and CD11c⁺CD86⁺ DCs in spleens. B Dot plots of CD68⁺CD206⁺ macrophages. C–E Percentage of CD11c⁺MHCII⁺ DCs, CD11c⁺CD86⁺ DCs, and CD68⁺CD206⁺ macrophages, respectively. Data were mean ± SD (n = 6, *p < 0.05, **p < 0.01, ***p < 0.001). p values were analyzed by one-way ANOVA followed by the LSD test