Fig. 6
IL-1β-primed ERCs decreased DKK1 secretion, but promoted β-catenin expression in splenic and colonic tissues. A The DKK1 level in supernatants of 3rd–7th-generation ERCs were detected by ELISA kit. The 5th generation of ERCs were recorded with the lowest DKK1 expression when compared with other generations (vs. the 5th generation of ERCs, *p < 0.05, **p < 0.01, ***p < 0.001). B DKK1 mRNA expression in P5 ERCs (n = 3). C DKK1 protein expression in P5 ERCs (n = 3). D DKK1 secretion in P5 ERC supernatants (n = 3). E–J The mRNA expression level of β-catenin in spleens (E) and colons (H) was detected, respectively. Furthermore, the protein level of β-catenin in spleens (F) and colons (I) was also demonstrated. Western blot analysis of β-catenin protein expression in the spleen (G) and colon (J) tissue homogenate. Data were presented as mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001). Statistical analysis was performed by one-way ANOVA followed by the LSD test