Fig. 3

Exosomal SNHG16 from CSCs promoted proliferation, migration and invasion. SHG44 and U251 cells were incubated with exosomes isolated from CSCs transfected SNHG16 or si-SNHG16 or its NC. A qRT-PCR analyzed the expression of SNHG16 in isolated exosomes. B The expression of SNHG16 in SHG44 and U251cells was determined. n = 6, *p < 0.05 vs CSC-NC or CSC-si-NC. C MTT was used to test viability of SHG44 and U251. n = 10, *p < 0.05 vs CSC-NC or CSC-si-NC. D, E Would healing assay to detect migration ability. Scale bar = 500 μm. n = 4, *p < 0.05 vs CSC-NC or CSC-si-NC. F, G Transwell assay to detect invasion ability. Scale bar = 200 μm. n = 4, *p < 0.05 vs CSC-NC or CSC-si-NC. H, I Colony formation assay to detect cell proliferation. Scale bar = 10 mm. n = 6, *p < 0.05 vs CSC-NC or CSC-si-NC