Fig. 2

SLC7A11 is a direct target of miR-375 in GC cells. A The volcano map indicating the number of mRNAs in SGC-7901 cells with or without miR-375 overexpression. B The mRNA heatmap showing the change of mRNA expression in SGC-7901 cells with or without miR-375 overexpression. C The venn map showing the potential targets of miR-375. D SLC7A11 protein expression is detected in GC cells with or without miR-375 overexpression. Data are presented as the mean ± SD, n ≥ 3, *p < 0.05, **p < 0.001 vs control. E The diagram indicating the binding of miR-375 on SLC7A11. F Luciferase reporter analysis was performed to determine the effect of miR-375 on the activity of SLC7A11 3′UTR WT and SLC7A11 3′UTR MUT. Data are presented as the mean ± SD, ns means no significance, n ≥ 3, *p < 0.05 vs NC+vector. G RIP assay was constructed to measure miR-375 level in RNA pulled down by Anti-Ago2 in GC cells with SLC7A11 3′UTR WT or SLC7A11 3′UTR MUT overexpression. Data are presented as the mean ± SD, n ≥ 3, **p < 0.01 vs pMIR-report empty group. H RNA-FISH was performed to evaluate the co-localization of miR-375 and SLC7A11 in GC cells