Fig. 5

MiR-375 attenuates the stemness of GC cells by targeting SLC7A11. A Online dataset (http://gepia.cancer-pku.cn/index.html) was used to detect SLC7A11 mRNA level in GC (T) and normal (N) tissues. B The correlation between SLC7A11 mRNA expression and the overall survival probability of GC patients was evaluated using the online analysis tool (http://kmplot.com). C, D The correlation between SLC7A11 mRNA expression and ALDH1A1 (C) or Oct4 (D) mRNA expression was evaluated using the online analysis tool (R2: Genomics Analysis and Visualization Platform (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi)). E CD44+ sub-population was determined in GC cells with miR-375 overexpression plus SLC7A11 overexpression or not. Data are presented as the mean ± SD, n ≥ 3, *p < 0.05, ***p < 0.001 vs LV3-NC+Vector, ^#p < 0.05, ^##p < 0.01 vs Vector+LV3-miR-375. F The protein expression of stemness markers was examined in the GC cells. G The sphere size and number were evaluated in the GC cells. H Tumor images derived from BGC-823 cells described in H. I Tumor weight of tumors shown in I. J Tumor images derived from SCG7901 cells described in J. K, L Tumor formation rate (K) and confidence intervals for 1/(stem cell frequency) (L) were calculated based on the result shown in L. Data are presented as the mean ± SD, n ≥ 3, *p < 0.05, **p < 0.01 vs NC