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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Submacular integration of hESC-RPE monolayer xenografts in a surgical non-human primate model

Fig. 1

Delivery of hESC-RPE graft from the production site to the subretinal space. A Phase contrast micrographs of RPE morphology before and after shipment. B TEER of hESC-RPE before and after shipment (mean and SD, from 5 independent shipments, 2–6 inserts per shipment). P > .05 (not significant, ns, Mann-Whitney U test). C Confocal micrographs of hESC-RPE grafts labeled post-shipment with antibodies against proteins important for RPE functionality (shown for the 54 h shipment). Scale bars, A 100 μm and C 20 μm. D–G Key surgical steps in submacular implantation of hESC-RPE grafts. D Triamcinolone-aided detachment of posterior cortical vitreous from the retinal surface. E Subretinal BSS injection to induce detachment of the macula. F Surgical removal of naive RPE with a custom-made extensible loop instrument (the overview is shown in the insert image). G Submacular implantation of hESC-RPE grafts on a porous polyester scaffold using a custom-made instrument

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