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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Submacular integration of hESC-RPE monolayer xenografts in a surgical non-human primate model

Fig. 6

Evaluation of retinal gliosis and in vivo functionality of the hESC-RPE cells. A1, B1, C1 Epifluorescent and brightfield micrograph overlays show only minor retinal GFAP upregulation, mainly around the retinotomy (B1) and in 4 of 6 cases also slightly in the fovea (B1, C1). A2, B2, C2 Vimentin is more upregulated with higher variation between animals than GFAP. A1, A2, B1, B2, C1, C2 Stains from 3 different animals. Both GFAP and vimentin are also upregulated in some areas under the graft (C1, C2). D, E Vimentin upregulation in ONL is observed in 2 of 8 animals. F–H Evaluation of the effectiveness of immunosuppression. F Epifluorescent and brightfield micrograph overlays show a few CD3-positive cells (arrowheads in the enlarged image indicated by the white box). The subretinal signal originates from POS. G Some amoeboid/activated Iba1-positive cells are present in the inner retina and subretinal space (arrowheads) but most reside in the choroid. H MHC-II positivity is detected occasionally in the retina (arrowheads), in pigmented cells in the subretinal space (asterisk), and in a few cells on the PET scaffold at the graft edge and in the choroid. I, J Confocal micrographs of ezrin immunolabeling identify microvilli at the apical surface of the hESC-RPE both prior to transplantations in vitro and after transplantation in vivo. K Confocal micrographs of rhodopsin immunolabeling show localization of POS inside the hESC-RPE cells compared with the monkey RPE. The nuclei counterstained with DAPI. L Confocal micrographs of unstained paraffin sections with excitation 488/568/647 nm show autofluorescence in some areas on the PET carrier in addition to monkey RPE. The image shows a central area of the hESC-RPE graft. Scale bars, A–H 200 μm, I–K (upper image) 20 μm, K (lower images) 5 μm, and L 50 μm

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