Fig. 3
From: Methylsulfonylmethane enhances MSC chondrogenic commitment and promotes pre-osteoblasts formation
Effects of MSM in the early phase of osteogenic differentiation. After 3 and 7 days of osteogenic differentiation a reduced number of differentiating cells was observed (A). In MSM-treated cells SP7 gene expression was upregulated after 3 and 7 days of differentiation whereas RUNX2 was downregulated after 7 days of differentiation (B). RUNX2 protein levels were similar after 3 days of osteogenic differentiation (C) and significantly lower after 7 days of differentiation (D) in nuclei of MSM treated cells. *p<0.05; **p<0.01