Fig. 2

Newborn TH+/BrdU+ neurons in the postnatal WT and wv mouse SNpc; effects of BNN-20. A,B Stereologically calculated numbers of total TH+ cells in the SNpc of wild-type (WT) and “weaver” (wv) mice that received BNN-20 or saline (sal) from P14 to P60, with co-administration of BrdU from P40 to P60 (in A), or from P20 to P40 (in B). Co-expression of BrdU is also shown (TH+/BrdU+ cells, in light blue/pink and TH+/BrdU− in dark blue/red) [Graph A: n = 3 per group, TH+/BrdU+ to total TH+ percentage comparison: a: p < 0.01 compared to WT sal. b: p < 0.05 compared to wv sal. c: p < 0.05 compared to WT BNN-20. Graph B: n = 3 per group, a: p < 0.001 compared to WT sal. b: p < 0.001 compared to wv sal. c: p < 0.001 compared to WT BNN-20. Statistical analysis was performed by two-way ANOVA followed by Bonferroni post hoc test (p < 0.001, F = 41.993 for drug, p < 0.001, F = 42.679 for genotype). Error bars are SEMs]. C,D Representative images after immunofluorescence stainings against TH and BrdU in the SNpc of C a cluster of TH+/BrdU+ cells observed in the SNpc of wv BNN-20 mice (fluorescence microscopy) and D in the SNpc of WT BNN-20 mice (confocal microscopy). Double-positive (TH+/BrdU+) newborn dopaminergic neurons are indicated by white arrows [scale bar= 50 μm]