Fig. 7
BNN-20 promotes neuronal differentiation of NSCs in vitro. A,B Dot plots showing the percentages of β-ΙΙΙ tubulin+ neurons in cultures of A wild-type (WT) SEZ-derived NSCs and B weaver (wv) SEZ- and SN-derived NSCs maintained in differentiation conditions for 5 days with or without (ctr) BNN-20 addition in the cell medium. C Representative immunofluorescence image of a cell culture stained for βIII tubulin [scale bar = 50 μm]. D,E Percentages of phosphohistone 3+ (PH3+) cells in cultures of D wild-type (WT) SEZ-derived and E weaver (wv) SEZ- and SN-derived NSCs, maintained for 5 days in proliferation conditions with or without (ctr) BNN-20 addition in the cell medium [Error bars are SDs. a: p < 0.05 using paired Student t-test analysis because the same cell samples were split in ctr or + BNN-20 conditions. n = 3 independent experiments]. F Representative immunofluorescence image of a cell culture stained for PH3 [scale bar= 50 μm]