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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: BMSC-derived extracellular vesicles intervened the pathogenic changes of scleroderma in mice through miRNAs

Fig. 1

Identification of mouse BMSCs and BMSC-derived EVs. a Morphology of BMSCs cultured in regular medium at passage 3. Scale bar 100 μm. b Oil Red O staining of BMSCs induced to undergo adipogenic differentiation for 21 days. Scale bar 200 μm. c Alizarin red staining of BMSCs induced to undergo osteogenic differentiation for 21 days. Scale bar 200 μm. d Toluidine blue staining of BMSCs induced to undergo chondrogenic induction for 21 days. Scale bar 200 μm. e Flow cytometry plots showing that the BMSCs were positive for CD44, CD105, CD90, and CD73, and negative for CD31, CD45. f Particle size distribution of BMSC-derived EVs measured by nanoparticle tracking analysis (NTA). g Morphology of EVs on transmission electron microscopy (TEM). h Western blot analysis of the protein profiles of EVs. BMSCs: bone marrow mesenchymal stem cells; EVs: extracellular vesicles derived from mouse BMSCs

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