Fig. 1
From: SiRNA in MSC-derived exosomes silences CTGF gene for locomotor recovery in spinal cord injury rats
Characterization of MSCs, MSC-Exo, and Exo-siRNA. a Morphology of MSCs under microscope. Bar = 50 μm. b The surface antigens CD29, CD90, and CD45 of MSCs were identified by flow cytometry. c TEM revealed the typical exosome structure of Exo and Exo-siRNA. The red arrow points to the Exo and Exo-siRNA. Bar = 200 nm. d The rat bone marrow MSCs marker Calnexin were analyzed by WB. e NTA analysis of Exo and Exo-siRNA showed the incorporation of siRNA did not affect the diameter of Exo. f The MSC-Exo marker proteins CD9 and CD63 were analyzed by WB