Fig. 2

Preliminarily analysis of silencing efficiency of three pairs of target siRNA. a Cy3-siRNA was transfected into HEK293T cells by Lipo3000, and the control group was not transfected. Bar = 100 μm. b The expressions of CTGF-related mRNAs at 72 h after siRNA transfection were presented. n = 3, data are represented as mean ± SD, *p < 0.05, **p < 0.01 vs control. c The absolute number of siRNA molecules in Exo under different electroporation conditions was exhibited. n = 3, data are represented as mean ± SD, *p < 0.05, **p < 0.01 vs group 6. d The loading rates of siRNA in Exo under different electroporation conditions were presented. n = 3, data are represented as mean ± SD, *p < 0.05, **p < 0.01 vs group 6. e Relative contents of naked siRNA and Exo-siRNA after RNase treatment were showed. n = 3, data are represented as mean ± SD, *p < 0.05, **p < 0.01 vs naked siRNA. f Fluorescence patterns of LPS/hypoxia-treated astrocytes after co-culture with PKH67 and Cy3 double-labeled Exo-siRNA for 72 h were assessed. Bar =10 μm