Fig. 4

Therapeutic effects of Exo-siRNA on SCI rats. Rats were classified into SCI group, Sham group, Exo group, and Exo-siRNA group, n = 6. a Fluorescence imaging of organs of Exo-siRNA group at 3 h, 24 h, and 48 h after intravenous injection was illustrated. b The results of H-E staining in the lungs of normal healthy rats in the Exo and Exo-siRNA group. Bars = 100 μm. c The relative enzyme activity of LDH, ALT, and SOD in rat liver. d Expressions of CTGF, GFAP, vimentin, fibronectin, and laminin were assessed by WB, n = 3, data are represented as mean ± SD, *p < 0.05, **p < 0.01 vs SCI group. e The relative level of CTGF, GFAP, vimentin, fibronectin, and laminin protein density. n = 3, data are represented as mean ± SD, *p < 0.05, **p < 0.01 vs SCI group. f Transverse sections of core lesion tissues in each group were stained by Masson’s trichrome method. Fibrotic tissue area was indicated by red arrows. Bars = 100 μm. g Relative quantification of glial scar area (%), n=3, data are represented as mean ± SD, *p < 0.05, **p < 0.01 vs SCI group