Fig. 4

Immunohistological and cell count results of Pou4f3DTR/+ mice at day 7 and day 14 after cell transplantation. A Schematic of the protocol of cell transplantation. B The graph shows the numbers of transplanted human cells in the organ of Corti and the spiral ganglion of the mouse cochlea at day 7 and day 14 after transplantation. n = 4 for day 7 and n = 3 for day 14. C The graph shows numbers of transplanted human cells in the scala tympani, scala vestibule, and scala media of the mouse cochlea at day 7 and day 14 after transplantation. n = 4 for day 7 and n = 3 for day 14. D Immunohistochemistry of the cochleae at day 7, day 14 after cell transplantation. Green indicates Cell-EGFP, white indicates Sox2, and blue indicates DAPI. Although most of the transplanted cells are in the scala tympani, some cells have migrated into the organ of Corti and express Sox2. Db’, b”, b”’, f’, f”, and f”’ show single channel for Db and Df, respectively. White arrows show the transplanted cells. Scale bars represent 50 μm. OC, organ of Corti; SG, spiral ganglion; DTR, diphtheria toxin receptor