Delivery method | Advantages | Disadvantages | Apparatus |
---|---|---|---|
Electroporation Nucleofection | High efficiency Less regulatory constraints cGMP compliant electroporation systems DNA, RNA, or plasmids | NK cells must be expanded and require cytokines Cell viability dependent on cargo (e.g., RNA, DNA) Cargo size affects efficiency | Neon Amaxa BTX MaxCyte |
Lipofection | Cost effective Readily available reagents DNA, RNA, or plasmids | Cell viability dependent on cargo (e.g., RNA, DNA) Limited studies Requires optimized conditions of reagents and cell medium | Lipofectamine 2000, 3000 |