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Table 1 Comparison of nonviral delivery strategies in NK cells

From: Nonviral genome engineering of natural killer cells

Delivery method

Advantages

Disadvantages

Apparatus

Electroporation

Nucleofection

High efficiency

Less regulatory constraints

cGMP compliant electroporation systems

DNA, RNA, or plasmids

NK cells must be expanded and require cytokines

Cell viability dependent on cargo (e.g., RNA, DNA)

Cargo size affects efficiency

Neon

Amaxa

BTX

MaxCyte

Lipofection

Cost effective

Readily available reagents

DNA, RNA, or plasmids

Cell viability dependent on cargo (e.g., RNA, DNA)

Limited studies

Requires optimized conditions of reagents and cell medium

Lipofectamine 2000, 3000