Fig. 2From: hPMSCs inhibit the expression of PD-1 in CD4+IL-10+ T cells and mitigate liver damage in a GVHD mouse model by regulating the crosstalk between Nrf2 and NF-κB signaling pathwayhPMSCs inhibit the expression of PD-1 and the apoptosis of CD4+IL-10+ T cells in vitro. a Gating strategy. Forward scatter (FSC) and side scatter (SSC) gating were used to discriminate viable cells from cell debris. In vitro, the CD4+IL-10+ T cells were defined as those positive for CD4 and IL-10, and PD-1+ cells were defined as those positive for PD-1. Furthermore, the apoptosis rate in PD-1+ and PD-1− cells was determined. b, c Representative dot plots of CD4+IL-10+ T cells and the percentage of CD4+IL-10+ T cells and the statistical evaluation of the levels of PD-1 in CD4+IL-10+ T cells from different groups of CD4+IL-10+ T cells. d The statistical evaluation of the apoptosis in different groups of CD4+IL-10+ T cells. e Dot plots from a representative experiment showing the apoptosis rate of PD-1+ and PD-1− cells and the correlation analysis of PD-1 expression and the apoptosis rate. The results were obtained from three independent experiments, *P < 0.05, **P < 0.01Back to article page