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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Plant callus-derived shikimic acid regenerates human skin through converting human dermal fibroblasts into multipotent skin-derived precursor cells

Fig. 5

Shikimic activates nestin expression via the MyD88/p38/CREB signaling pathway. a After the designated incubation time with shikimic acid, the phosphorylation level of MAPK-kinase (MKK)3/6, p38 MAPK, and CREB was determined (upper). Quantification of the obtained results (N = 3 in each group, *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control) (lower). b Western blot analysis of nestin, fibronectin, and vimentin after treatment of MyD88 inhibitor and shikimic acid. c Western blot analysis of nestin, fibronectin, and vimentin after treatment of p38 inhibitor and shikimic acid. d Chromatin immunoprecipitation (ChIP) assay on the promoter of nestin. Phosphor-CREB antibody was used for the ChIP assay (N = 3 in each group, *p < 0.05)

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