Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Placental chorionic plate-derived mesenchymal stem cells ameliorate severe acute pancreatitis by regulating macrophage polarization via secreting TSG-6

Fig. 6

CP-MSC-derived TSG-6 played a vital role in inducing M2 polarization of macrophages and reducing pancreatic injury and systemic inflammation in SAP rats. A Flow cytometric analysis of the macrophage polarization phenotype after CP-MSCs (TSG-6 shRNA) or CP-MSCs (scr shRNA) co-cultured with M1 macrophages for 24 h. B Quantification of macrophage polarization phenotype. CD86+ macrophages are considered as M1 macrophages, and CD163+ macrophages are considered as M2 macrophages (n = 3). C The mRNA expression levels of M1 marker genes (IL1-β, TNF-α, and iNOS) in macrophages (n = 3). D The mRNA expression levels of M2 marker genes (Arg1, IL-10, and CD163) in macrophages (n = 3). TSG-6 protein (E) and mRNA (F) expression levels in CP-MSCs (TSG-6 shRNA), CP-MSCs (scr shRNA), and CP-MSCs (n = 3). G Representative immunofluorescence staining of the polarized phenotype of macrophages in the pancreas and liver tissue. Scale bars, 50 μm. H Quantification of M2 macrophages (CD163+) and M1 macrophages (iNOS+) in the pancreas and liver tissue. The numbers of immunopositive cells represent the mean ± SEM of at least 5 fields (n = 5). I H&E staining of pancreas tissue. Scale bars, 50 μm. J RT-qPCR analysis of TSG-6 expression level of CP-MSCs after intervention by different concentrations of SAP rat serum (n = 3). K RT-qPCR analysis of the TSG-6 expression level of CP-MSCs after CP-MSCs were co-cultured with M1 macrophages or M2 macrophages for 24 h (n = 3). L Histopathological scores for the pancreas showed the degree of edema, inflammation infiltrate, hemorrhage, and acinar necrosis (n = 5). M The mRNA expression levels of pro-inflammatory factors (IL-1β and TNF-α) and anti-inflammatory factors (IL-4 and IL-10) in the pancreas (n = 5). N The concentrations of pro-inflammatory factors (IL-1β and TNF-α) and anti-inflammatory factors (IL-4 and IL-10) in rat serum (n = 5). Data are presented as mean ± SEM. Significance is indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001; NS, no significant

Back to article page