Fig. 1
From: Temporal induction of Lhx8 by optogenetic control system for efficient bone regeneration
Lhx8 showed dynamic patterns of expression during osteogenic differentiation and promoted cell proliferation. a Relative mRNA expression of Lhx8 during the osteogenic induction of rat BMSCs in vitro. b qPCR analysis showed that the expression of Lhx8 mRNA was significantly increased in the over Lhx8 group. BMSCs transfected with lentivirus overexpressing Lhx8 and control respectively. c, d The protein expression of Lhx8 was evaluated by western blotting. e CCK-8 growth curves showed that Lhx8 promoted the cell viability of BMSCs. f, g Representative images and quantitative analysis of EdU staining (green) of BMSCs in the two groups. Nuclear DNA was counterstained with Hoechst. Scale bar=50μm. All experiments were performed in triplicates. ***P<0.001, ****P<0.0001 vs. Ctrl