Fig. 2
From: Temporal induction of Lhx8 by optogenetic control system for efficient bone regeneration
The sustained expression of Lhx8 inhibited the osteogenic differentiation of BMSCs. a ALP activity was detected by colorimetric assay in the cell lysates of BMSCs on day 0, 3, 7, and 14 under osteogenic induction. b Gross appearance of ALP staining is shown in the upper image while the microscopic appearance is shown in the lower image. Scale bar=400μm. c–f The mRNA expression of osteogenic-specific genes (ALP, Runx2, OSX, OCN) after osteogenic induction on days 3, 7, and 14 day. g The protein expression of osteogenic-specific genes (ALP, Runx2, OSX, OCN) after osteogenic induction on day 0, 3, 7, and 14. The quantitative western blot results were shown above the bands. h Gross appearance of Alizarin red staining performed after 21 days of osteogenic induction is shown in the upper image while the microscopic appearance is shown in the lower image. Scale bar=400μm. All experiments were performed in triplicate. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 vs. Ctrl