Fig. 3
From: Temporal induction of Lhx8 by optogenetic control system for efficient bone regeneration
An optogenetic control system for the optimization of Lhx8 expression towards efficient osteogenesis in vitro. a Schematic representation of the optogenetic regulation system for Lhx8. Transactivation of Lhx8 was activated by blue light stimulation. b, c Representative images and quantitative analysis of EdU staining (green) of BMSCs in the two groups. Nuclear DNA was counterstained with Hoechst. Scale bar=50μm. d Schematic representation of grouping and the experimental procedure. e Representative images of gross appearance (upper) and microscopic images (lower) with ALP staining after 7 days of osteogenic induction. Scale bar=300μm. f–h The mRNA expression of osteogenic-specific genes (ALP, Runx2, OCN) after osteogenic induction on day 7. i The protein expression of osteogenic-specific genes (ALP, Runx2, OCN) after osteogenic induction on day 7. The quantitative western blot results were shown above the bands. j Representative images of gross appearance (upper) and microscopic images (lower) with Alizarin red S staining of calcified nodules after 21 days of osteogenic induction. Scale bar=100μm. k Semi-quantitative evaluation of Alizarin Red S staining by CPC. All experiments were performed in triplicates. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 vs. Ctrl