Fig. 3
Survival, weight changes, and bone marrow engraftment after co-transplantation of BMCs and TMSCs. a The mRNA expression levels of MMP3 in control and MMP3-knockdown TMSCs. GAPDH was used as the reference gene. b MMP3 protein concentrations in control TMSC-CM and MMP3-knockdown TMSC-CM were quantified using a human MMP3 ELISA kit. c Experimental scheme: BALB/c mice were treated with the Bu/Cy regimen, transplanted after 48 h, and sacrificed 10 or 24 days after BMT. d Mouse survival was measured using Kaplan-Meier estimator and statistical analysis was performed using log-rank test (**P < 0.01). e Body weight changes from day − 7 to day 24 after transplantation. The data represent the mean ± S.E.M. and were analyzed using two-way ANOVA (*P < 0.05, compared with weights of the Bu/Cy group on the day specified). f Analyses of MHC haplotype expression in bone marrow cells on day 1 and g blood mononuclear cells on day 10 using flow cytometry. H-2d for donor C57BL/6 and H-2b for BALB/c recipient. h The numbers of RBCs and WBCs in peripheral blood were examined using Auto Hematology Analyzer. i The representative histology of the femur medullary cavity stained with H&E. Bone marrow sections were obtained from mice sacrificed on days 10 and 24. Reconstituted areas of the bone marrow are indicated by the gray arrows. Magnification × 20. j The bone marrow cellularity of the medullary cavity of the femur diaphysis was calculated. Data were analyzed using one-way ANOVA (*P < 0.05, **P < 0.01; ***P < 0.001)