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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Bone marrow-derived mesenchymal stem cells combined with gonadotropin therapy restore postnatal oogenesis of chemo-ablated ovaries in rats via enhancing very small embryonic-like stem cells

Fig. 8

Representative photomicrographs of PCNA immune-stained sections of the different experimental groups. a Group I (control group) showed a negative nuclear reaction in the surface epithelial cells (thin arrow) and stromal (S) cells and a positive reaction in the primary oocytes of primordial follicles (arrowheads) and the follicular cells (F). b Group II (OA group) showed negative reaction in the surface epithelial cells (thin arrow), follicular (F), and stromal (S) cells. c Group III (OA + PMSG group) showing an intense reaction in the stratified surface epithelial cells (thin arrows) with a negative reaction in the follicular (F) and stromal (S) cells. d Group IV (OA + BM-MSCs group) showed a negative reaction in the surface epithelial cells (thin arrow) and stromal(S) cells and a positive reaction in the follicular (F) cells. e Group V (OA + PMSG+ BM-MSCs group) showed a positive reaction in the surface epithelial cells (thin arrow), primary oocytes of the primordial follicles (arrow heads), and follicular (F) cells with a negative reaction in the stromal (S) cells. f Histogram representing the mean area percentage of PCNA immunoreaction in all experimental groups. The significant differences against chemo-ablated group indicated by a “*” for p < 0.05 and a “**” for p < 0.01. Data are shown as mean ± S.E.M, n = 7

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