Fig. 5

EpSC-seeded PCL+CA micro/nanofiber scaffolds can promote wound healing through activation of the Notch pathway. a Process map of whole animal experiment. b Representative images of wounds from each group taken at 0 day, 3 days, 7 days, 14 days, and 21 days post-injury. c Wound areas are shown for the control group, empty scaffold group, and EpSC scaffold group wounds. The wound healing rate was calculated by dividing the current area by the initial area. d Masson staining and PAS staining of neoskin taken 21 days post-injury. Scale bar = 100 μm (Masson staining), 50 μm (PAS staining). e Immunofluorescence staining of cytokeratin 19 (green) in neoskin taken 21 days post-injury. Scale bar = 50 μm. f Representative western blot images and densitometric analysis results showing the expression levels of the Notch pathway-related proteins Notch1, Jagged1, and Hes1 in neoskin sampled 21 days postinjury from each group. *P<0.05, **P<0.01, ***P<0.001 vs control group