Fig. 2
From: The stemness of hepatocytes is maintained by high levels of lipopolysaccharide via YAP1 activation
LPS promotes colony formation and pluripotent marker expression in hepatocytes. a Left: Representative images of AML12 cells treated with or without LPS; right: quantification of colony diameters. Scale bars, 100 μm. b Left: Representative images of colony formation in AML12 cells after culture with or without LPS for 12 days; right: quantification of colony numbers. c The relative mRNA expression of pluripotent and stem cell markers in AML12 cells cultured in basic medium in the presence or absence of LPS for 24 h. mHPCs were used as a positive control. d Western blot assay for Sox2, cMyc, Klf4, and Sox9 in AML12 cells cultured in basic medium containing LPS for the indicated amounts of time. h: hours; d: days. **P < 0.01, ***P < 0.001