Fig. 2
From: SOX9 inactivation affects the proliferation and differentiation of human lung organoids
SOX9−/− does not affect the maturation of AT1 and AT2 cells. A Representative bright-field images of SOX9 wild-type (WT) and knockout (SOX9−/−) organoids on day 31 or 65. Scale bars, 500 μm. B mRNA expression levels of AT2 markers (SP-B, SP-C, and LAMP3) and AT1 marker (AGER, AQP5, PDPN) in organoids on days 31 and 65 relative to H9 hESCs. Data represent mean ± SEM (n = 3 independent experiments). C, D Immunofluorescence labeling of AT2 markers (SP-C) and AT1 markers (PDPN and AQP5) in organoids on day 31 (C) and day 67 (D). Scale bars, 50 μm