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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism

Fig. 3

Pro-angiogenic effects of hEMSCs in vitro. a Representative micrographs from the Matrigel tube formation assay. The number of tubes formed was significantly higher in hEMSCs than hBMSCs, n = 6/group. Real-time qPCR and Western blotting were performed to measure the level of mRNA and protein expression of VEGFA (b, e, f), angiopoietin 1 (ANG1, c, e, g), and angiopoietin 2 (ANG2, d, e, h). Representative Western blot images (e, i, l) and quantification of VEGFA (f), ANG1 (g), ANG2 (h), Fms-like tyrosine kinase 1 (FLT-1, j), TIE2 (k), transforming growth factor β2 (m), and FGF9 (n) protein levels in hEMSCs or hBMSCs. Data are expressed as mean ± SEM. n = 5 or 6/group for all the real-time qPCR and Western blotting quantifications

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Stem Cell Research & Therapy

ISSN: 1757-6512