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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Aldo-keto reductase family 1 member C1 regulates the osteogenic differentiation of human ASCs by targeting the progesterone receptor

Fig. 2

Knockdown of AKR1C1 enhanced the osteogenic capacity and impaired the adipogenic capacity of hASCs in vitro. a Transfection efficiency showed by fluorescence microscopy. b Knockdown efficiency of AKR1C1 determined by qRT-PCR and western blot. c Knockdown of AKR1C1 enhanced the ALP activity after 7 days of osteogenic induction as shown by ALP staining and quantification. d Knockdown of AKR1C1 accelerated mineralization after 21 days of osteogenic induction as shown by ARS staining and quantification. e Knockdown of AKR1C1 promoted the mRNA expression of RUNX2 on the 7th day of osteogenic induction and BGLAP on the 14th day of osteogenic induction as determined by qRT-PCR. f The protein expression of RUNX2 tested by western blot was consistent with the result of qRT-PCR. g Knockdown of AKR1C1 inhibited lipid droplet formation after 21 days of adipogenic induction as shown by oil red O staining. h Knockdown of AKR1C1 inhibited the mRNA expression of PPARγ and CEBPα on the 7th day of adipogenic induction as determined by qRT-PCR. i The protein expression of PPARγ tested by western blot was consistent with the result of qRT-PCR. **p < 0.01, ***p < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512