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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Aldo-keto reductase family 1 member C1 regulates the osteogenic differentiation of human ASCs by targeting the progesterone receptor

Fig. 5

Knockdown of AKR1C1 promoted osteogenesis of hASCs in vitro through targeting PGR. a, b AKR1C1 knockdown inhibited expression of PGR but not AR validated by qRT-PCR and western blot. c, d QRT-PCR and western blot indicated that overexpression of AKR1C1 by wild type plasmid (WT) in the AKR1C1 knockdown cells upregulated expression of PGR whereas mutant plasmids (E127D, H222I, and R304L) had no significant influence. e ARS staining and quantification showed PGR knockdown in ASCs accelerated mineral accumulation after 21 days of osteogenic induction. f, g QRT-PCR and western blot showed that PGR knockdown in ASCs promoted expression of RUNX2 and BGLAP after 14 days of osteogenic induction. h Expression of PGR in AKR1C1 knockdown cells slightly downregulated the ALP activity. i Expression of PGR in AKR1C1 knockdown cells slowed down mineral accumulation. j, k QRT-PCR and western blot showed that expression of PGR in AKR1C1 knockdown cells downregulated the expression of osteogenic markers (RUNX2, 7 day and BGLAP, 14 day). *p < 0.05, **p < 0.01, ***p < 0.001

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