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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Nicotinamide promotes pancreatic differentiation through the dual inhibition of CK1 and ROCK kinases in human embryonic stem cells

Fig. 1

Enhancement of pancreatic progenitor induction by nicotinamide depends on kinase inhibition, but not on PARP and SIRT regulation. a Four-stage differentiation strategy to induce pancreatic progenitors from hESCs; b RT-qPCR analysis for mRNA levels of NKX6.1 and PDX1 in cells on day 13 of differentiation, treated with nicotinamide (n = 3), *p < 0.05; c Nicotinamide inhibits PARP, SIRT and several kinases, and contributes to NAD synthesis; d RT-qPCR analysis for mRNA level of NKX6.1 and PDX1 in cells on day 13 treated with nicotinamide (10 mM), Y27632 (10 μM), D4476 (5 μM), niacin (5 mM), PARP inhibitor ABT888 (50 nM) and SIRT inhibitor EX527 (10 μM) (n = 3), *p < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512