Fig. 2From: Nicotinamide promotes pancreatic differentiation through the dual inhibition of CK1 and ROCK kinases in human embryonic stem cellsGeneric chemical inhibition of CK1 promotes pancreatic progenitor induction. a RT-qPCR analysis of pancreatic progenitors on day 13 treated with nicotinamide and D4476 to measure mRNA levels of NKX6.1, PDX1, PTF1A and SOX9 (n > 3), *p < 0.05; b Immunostaining for PDX and NKX6.1 expression in pancreatic progenitors on day 13 under Mock, nicotinamide and D4476 conditions, scale bar 100 μm (n = 3); c Flow cytometry analysis of pancreatic progenitors on day 13 for PDX1+ and NKX6.1+ percentage under Mock, nicotinamide and D4476 conditions (n = 3); d, d` Western blot analysis of p-β-Catenin (Ser45) in pancreatic progenitors on day 13 under Mock, nicotinamide, D4476 and Y27632 conditions (n = 3), *p < 0.05Back to article page