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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Mesenchymal stem cells ameliorate renal fibrosis by galectin-3/Akt/GSK3β/Snail signaling pathway in adenine-induced nephropathy rat

Fig. 1

Diagram of iTRAQ proteomics experimental design. Male Sprague Dawley rats were induced by adenine gavage for 20 days and MSCs treatment for 5 days. Kidney tissues were collected, protein extracted, pooled before iTRAQ labeling, and subjected to mass spectrometric analysis. Eight iTRAQ labels were used: 113 and 114 for A1 and A2; 115,116, and 117 for B1, B2, and B3; and 118, 119, and 121 for C1, C2, and C3, respectively. Bioinformatics analysis (GO, KEGG Pathway) was used to examine differential expression proteins. Mass spectrometry results were further confirmed by assessing galectin-3 protein with western blot and Q-PCR in kidney tissues and in NRK-52E and NRK-49F induced by human recombinant TGFβ1

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