Fig. 5

MSCs treatment alleviated the tubulointerstitial fibrosis in adenine-induced rats. Immunohistochemistry of collagen type I (A), collagen type II (B), collagen III type (C), and α-SMA (D) in the tubulointerstitium. Representative immunohistochemical staining images of collagen type I-, II-, III-, and α-SMA positive areas in kidney sections at 5 days post-MSC treatment (scale bar, 100 μm). The statistical graph shows the integrated optical density (IOD) of collagen type I-, II-, III-, and α-SMA positive areas (n = 6-8 in each group) by the Image Pro Plus 6.0 software. N = 5 (per group), data are presented as mean ± SD, analyzed by one-way ANOVA followed by Bonferroni post hoc testing. *P < 0.05, vs. control group, ^#P < 0.05 vs. adenine group