Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Exosome-transported circRNA_0001236 enhances chondrogenesis and suppress cartilage degradation via the miR-3677-3p/Sox9 axis

Fig. 3

Exosomal circ_0001236 validation and expression in OA and healthy cartilage chondrocytes. a The presence of exosomal circ_0001236 was validated in primary human chondrocytes by RT-PCR. Divergent primers amplified circ_0001236 from cDNA, but not from genomic DNA. GAPDH was used as a negative control. b Schematic illustration showing the circularization of ZC3H78 exons 12 and 13 to form circZC3H78. The presence of circ_0001236 was validated by RT-PCR, followed by Sanger sequencing. c Northern blot analysis for the detection of circZC3H78 and ZC3H78 expression in chondrocytes treated with or without RNase R. d–f RNA FISH revealed the predominant localization of circ_0001236 in the cytoplasm. circ_0001236 probes were labeled with Cy-3. The nuclei were stained with DAPI (scale bar, 20 μm). g circ_0001236 expression was higher in healthy cartilage than in OA cartilage tissues (scale bar, 50 μm). The experiment was repeated independently three times, and representative results are shown

Back to article page