Table 2 Primer sequences used for qPCR
Gene | Primer sequence forward 5′–3′ | Primer sequence reverse 5′–3′ |
|---|---|---|
Pecam1 | TTGGCACCATGAACAAACTAGCA | CGCTTCGGAGACTGGTCACA |
CD34 | TGCCGTCTGTCAATGTTTCTGATTA | TCGGATTCCTGAACATTTGATGTC |
Kdr | AATGCCCATGACCAAGAATGTG | GGATAGAGCCGCGTGTCTGAA |
Nos 2 | CTCACTGTGGCTGTGGTCACCTA | GGGTCTTCGGGCTTCAGGTTA |
Nos 3 | GCGGCTGGTACATGAGTTCAGA | AGATCCATGCAGACAGCCACA |
TIMP-2 | GACACGCTTAGCATCACCCAGA | CTGTGACCCAGTCCATCCAGAG |
MMP-14 | GAGAACTTCGTGTTGCCTGATGAC | TTCTGGGCTTATCTGGGACAGAG |
GAPDH | GGACCAGGTTGTCTCCTGTG | CACCTGGAGTACCGGATGT |