Fig. 1

The 3D self-assembling peptide induction system followed by the OP9 coculture system promotes hematopoietic differentiation of mouse PSCs. A Schematic overview of mouse PSCs hematopoietic differentiation in the 3D self-assembling peptide induction system followed by the OP9 coculture system. B Representative morphology of hematopoietic cells from hematopoietic-like colonies derived from EBs in a 3D induction system followed by coculture with OP9 stromal cells and 0.1% gelatin. C Representative flow cytometry analysis of the percentage of Lin⁻Sca-1⁺c-kit⁺(LSK) on day 5 and day 7 respectively (n = 3). D Statistical analysis of percentage of mesoderm cells (Flk1⁺), hemogenic endothelium (TIE2⁺c-kit⁺), Lin⁻c-kit⁺ and LSK on day 2, day 5, and day 7 respectively (n = 3). E Absolute numbers of Flk1⁺, TIE2⁺c-kit⁺, Lin⁻c-kit⁺, and LSK cells in between 3D+OP9 and 3D+0.1% gelatin on day 2, day 5, and day 7 respectively (n = 3). F Representative statistically enriched pathways of the upregulated genes in 3D+OP9 group compared with 3D+0.1% gelatin group. Data are the means ± SD from three independent experiments. Error bars represent mean ± SD of samples from three independent experiments (n = 3). NS, not significant; *p < 0.05, **p < 0.01, ***p < 0.001