Fig. 5

Differentiated D5-LSKCD201⁺ cells showed more hemogenic- and hematopoietic-related markers, similar to those of HSCs, than D5-LSKCD201⁻ cells. A Volcano plot for CD201⁺ cells and CD201⁻ cells in LSK cells on day 5 of our coculture differentiation system. The green and red dots represent 2252 and 2193 upregulated genes in the CD201⁺ and CD201⁻ cells, respectively (q< 0.05). B Representative statistically enriched pathways of the differentially expressed genes in CD201⁺ and CD201⁻ cells in LSK cell population. C The expression level of arterial-related genes determined by RNA-seq in CD201⁺ cells compared with that in CD201⁻ cells is shown as FPKM values. Data are represented as mean ± SD (n = 3). D The expression level of endothelia and EHT specific genes in CD201⁺ cells compared with that in CD201⁻ cells is shown as FPKM values. Data are represented as mean ± SD (n = 3). E The expression level of lymphoid and myeloid-erythroid biased regulator genes in CD201⁺ cells compared with that in CD201⁻ cells is shown as the FPKM values. Data are represented as mean ± SD (n = 3). Error bars represent mean ± SD of samples from at least three independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001