Fig. 2

Effects of hAMSCs-CM on HFD-induced obesity in mice. a Mice were fed with HFD or CHOW for 15 weeks and were intraperitoneally injected with hAMSCs-CM suspended in 0.4 mL serum-free DMEM (SFD) (for the hAMSCs-CM group) or 0.4 mL SFD alone for the HFD group every week and their body weight were monitored every week. b, c Intraperitoneal insulin tolerance test (IPITT, right) and intraperitoneal glucose tolerance test (IPGTT, left) were performed at ninth or tenth week, respectively. Blood glucose concentration was determined by chemstrips and a glucometer through tail vein. d Weights of eWAT and iWAT were quantitatively analyzed. e Images of WAT and BAT were analyzed by HE staining (scale bars, 50 μm). f, g Sizes of adipocytes were quantitatively analyzed in WAT (f) and BAT (g). Serum total cholesterol (TC, h), alanine transaminase (ALT, i), aspartate aminotransferase (AST, j), low-density lipoprotein cholesterol (LDL-C, k), and triglycerides (TG, l), total protein (TP, m), creatinine (CREA, n) and blood urea nitrogen (o, BUN) were measured using automatic biochemical analyzer (Beckman Coulter AU2700). Data represent as means ± SD. #p< 0.05, ##p< 0.01, ###p< 0.001 and *p< 0.05, **p< 0.01, ***p< 0.001 represents CHOW-D vs HFD and HFD vs HFD-CM, respectively