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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Human amniotic mesenchymal stem cells-conditioned medium protects mice from high-fat diet-induced obesity

Fig. 4

Effects of hAMSCs-CM on the expressions of the proteins related with lipid and glucose metabolism, and thermogenesis in multiple tissues of obese mice. a Expressions of GLUT4 in liver, PPARα and GLUT4 in WAT&BAT&muscle, and PGC-1α in WAT were examined by western blot. b–e The protein expressions of GLUT4 in the liver (b) & WAT (c) & BAT (d) & muscle (e), PPARα in WAT (c) & BAT (d) & muscle (e), and PGC-1α in WAT (c) were quantitatively analyzed. f–h the protein expressions of P-AMPK and AMPK in WAT were determined by western blot (f) and quantitative analyses (g, h). i–k The protein expressions of P-AKT and AKT in WAT and liver were determined by western blot (i) and quantitative analyses (j, k). l–n the protein expressions of P-GSK3β and GSK3β in the liver were determined by western blot (l) and quantitative analyses (m, n). o UCP1 expression was determined by immunofluorescent staining in BAT (scale bars, 20 μm). p, q The expression of UCP1 protein was determined by western blot (p) and quantitative analysis (q) in BAT. r UCP1 mRNA expression was also examined by RT-PCR in BAT. β-actin was served as the loading control. Data represent as means ± SD. *p< 0.05; **p< 0.01, ***p< 0.001

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