Fig. 4

NONFH exosomes could lead to NONFH-like damage on rats. A COR, TRA, SAG, MPR, and 3-DR images of rat femoral heads. COR, coronal; TRA, transverse; SAG, sagittal; MPR, multiplanar reconstruction; 3-DR, three-dimensional reconstruction. B Quantitative analysis of micro-CT scanning. BV/TV, bone volume per tissue volume; Tb.N, trabecular number; Tb.Sp, trabecular separation; Tb.Th, Trabecular thickness. C H&E staining of the femoral heads (scale bar = 200 μm). D Quantitative analysis of marrow cavity. E The expression of RUNX2 and CD31 in the femoral head of rats were measured by IHC staining (scale bar = 50 μm). F Western blot was used to detect the expression of collagen type 1, VEGFA, FGF2, RUNX2, OPN, PPARγ, ALP, and OCN in femoral heads of rats. *P < 0.05, versus PBS group; #P < 0.05, versus FNF exosome group. All data were expressed as mean ± SEM