Fig. 1

MTT assay for evaluating the impact of double overexpression of microRNAs on against the oxidative stress damage and qPCR. A By 6 h after cell culturing, the result of MTT assay, * vs. other groups with different symbols (†, ‡), p< 0.0001. B By 24 h after cell culturing, the result of MTT assay, * vs. other groups with different symbols (†, ‡), p< 0.0001. C By 48 h after cell culturing, the result of MTT assay, * vs. other groups with different symbols (†, ‡), p< 0.0001. n=8 for each group. G1= iPS-MSC; G2 = iPS-MSC^dOex-mIRs; G3 = iPS-MSC + H₂O₂/100uM; G4 = iPS-MSC^dOex-mIRs + H₂O₂/100uM. D The qPCR analytical result of relative expression of miR-19a-3p in iPS-MSC with and without H₂O₂ treatment, * vs. †, p< 0.01. E The qPCR analytical result of relative expression of miR-19a-3p in iPS-MSC^dOex-mIRs with and without H₂O₂ treatment, * vs. other groups with different symbols (†, ‡), p< 0.0001. F The qPCR analytical result of relative expression of miR-20a-5p in iPS-MSC with and without H₂O₂ treatment, * vs. †, p< 0.01. G The qPCR analytical result of relative expression of miR-20a-5p in iPS-MSC^dOex-mIRs with and without H₂O₂ treatment, * vs. other groups with different symbols (†, ‡), p< 0.0001. n=4 for each group. dOex-mIRs = overexpression of double microRNAs (i.e., miR-19a-3p and miR-20a-5p). All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡) indicate significance (at 0.05 level)