Fig. 4

In vitro analysis of protein expressions of apoptosis and fibrosis cellular level of senescence. A1, A2 Protein expression of cleaved caspase 3 (c-Casp3), * vs. other groups with different symbols (†, ‡), p< 0.001. B1, B2 Protein expression of cleaved Poly (ADP-ribose) polymerase (c-PARP), * vs. other groups with different symbols (†, ‡), p< 0.001. C1, C2 Protein expression of Smad3, * vs. other groups with different symbols (†, ‡), p< 0.001. D1, D2 Transforming growth factor (TGF)-ß, * vs. other groups with different symbols (†, ‡), p< 0.001. E–H Illustrating the immunofluorescent microscopic finding (400×) for identification of positively-stained β-galactosidase cells (blue color). I Analytical result of anumber of β-galactosidase+ cells, * vs. other groups with different symbols (†, ‡), p< 0.001. Scale bars in the lower right corner represent 20 μm. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n=4). Symbols (*, †, ‡) indicate significance (at 0.05 level). G1= iPS-MSC); G2 = iPS-MSC^dOex-mIRs; G3 = iPS-MSC + H₂O₂/100uM; G4 = iPS-MSC^dOex-mIRs + H₂O₂/100uM