Fig. 1
From: Adipose-derived stem cells therapy effectively attenuates PM2.5-induced lung injury
Adipose-derived stem cell transplantation alleviated PM2.5-induced inflammation and pulmonary fibrosis. C57BL/6 mice were administered PBS or 10 mg/kg PM2.5 every other day via intratracheal instillation for 4 weeks. At the fourth and sixth weeks, some of the PM2.5-exposed mice were treated with adipose-derived stem cells (ADSCs) via tail vein injection (2 × 105 cells/mouse). A Schema of the experimental procedure. B–E After mice were sacrificed, serum TNFα (B), IL-1β (C), and IL-6 (D) levels and the mRNA levels of pulmonary inflammatory and fibrotic genes (E) were measured. F Representative lung sections were stained with hematoxylin and eosin (H&E) stain, Masson’s trichrome stain, and antibodies specific for neutrophils and macrophages (galectin-3, Gal-3) (brown staining). Scale bar = 100 μm. G–I The relative collagenous fiber area (G) and the numbers of neutrophils (H) and Gal-3-positive cells (I) were quantified. N = 5; the data are presented as the mean ± SD; asterisk indicates p < 0.05; double asterisks indicate p < 0.01