Fig. 2

Comparation of multilineage differentiation potential and cytokine expression in HD-MSCs and AML-MSCs. a, b Adipogenic differentiation potential of HD-MSCs and AML-MSCs identified by Oil Red O staining (a) and qRT-PCR analysis of adipogenic differentiation-associated genes (ADIPOQ, PPAR-γ) (b). c, d Osteogenic differentiation potential of HD-MSCs and AML-MSCs identified by Alizarin Red S staining (c) and qRT-PCR analysis of osteogenic differentiation-associated genes (RUNX2, BGLAP) (d). e, f Chondrogenic differentiation potential of HD-MSCs and AML-MSCs identified by Alician Blue staining (e) and qRT-PCR analysis of chondrogenic differentiation-associated genes (SOX9, ACAN) (f). g Quantitative analysis of representative cytokines and inflammatory factors (COX2, VEGF, IL-10, IL-8, IL-6, TGF-β, HGF) in HD-MSCs and AML-MSCs. Scale bar=200 μm. All data were shown as mean ± SEM (n = 4). *P < 0.05; **P < 0.01; NS, not significant