Fig. 4

Validation of the common gating strategy. A multicenter analysis was performed to validate the common gating strategy. Fourteen AT samples were analyzed by flow cytometry with the two antibody panels, seven in AP-HM facilities and seven in SSCF facilities. The analysis was performed by the same operator using the common gating strategy. Each color represents a batch. Squares represent experiments realized in SSCF facilities. Rounds represent experiments realized in AP-HM facilities. No difference could be observed in cell subset distribution. EC, endothelial cell; ASC, adipose-derived stromal cell; Leuk, leukocytes; PR, pericyte