Fig. 6

miRNA-19b-3p promoted the osteogenic differentiation of BMSCs. A Expression of 10 miRNAs related to osteogenesis in serum exosomes from young rats; high expression of miR-19b-3p, miR-20a-5p, miR-21-5p, and miR-26b-5p was detected. B Transfection efficiency of the miR-19b-3p mimic. C BMSCs were stained for ALP after induction. D Conserved seed sequence of miR-19b-3p in the 3′UTR of PTEN mRNA. E After miRNA-19b-3p mimic transfected, the mRNA expression levels of ALP, Collagen I, and RUNX2 in BMSCs were increased, whereas that of the predicted gene PTEN was decreased. F WB analysis of the levels of PTEN in BMSCs treated with the miR-19b-3p mimic and nc. The BMSCs of the mimic group expressed higher levels of Collagen I and lower levels of PTEN. The data are expressed as the mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ****P < 0.0001